Nucleic acids - protein interactions
2014
2020
The interests of our laboratory lie in understanding protein-nucleic acids interactions that play roles in telomere DNA/chromatin remodeling, non-coding RNA recognition, and bacterial toxin-antitoxin systems’ assembly and functions. We employ biophysical methods that include solution state NMR, CD, fluorescence spectroscopy, X-ray crystallography, isothermal calorimetry, computational analysis, and biochemical assays in our work.
Representative publications:
Please see ‘Publications’ page for the complete list of publications.
Please see ‘Research’ page for more information on current projects in the lab.
Representative publications:
- Kumar, N., Taneja, A., Ghosh, M., Rothweiler, U., Sundaresan N.R., and Singh, M.* (2024) Harmonin homology domain-mediated interaction of RTEL1 helicase with RPA and DNA provides insights into its recruitment to DNA repair sites. Nucleic Acids Research. 2024, Feb 9;52(3):1450-1470. doi: 10.1093/nar/gkad1208.
- Manikandan, P., Sandhya, S., Paul S., Srinivasan N., Rothweiler U.*, and Singh, M.* (2022) Identification, functional characterization, assembly, and structure of ToxIN type III toxin-antitoxin complex from E. coli. Nucleic Acids Research. Feb 22;50(3):1687-1700. doi: 10.1093/nar/gkab1264.
- Jadhav, P.V.$, Sinha, V.K$., Chugh, S., Kotyada, C., Bachhav, D., Singh, R., Rothweiler, U. *, and Singh, M.* (2020). 2.09 Å resolution structure of E. coli HigBA toxin-antitoxin complex reveals an ordered DNA-binding domain and intrinsic dynamics in antitoxin. Biochemical Journal. Oct 30;477(20):4001-4019. doi: 10.1042/BCJ20200363.
- Ghosh, M. and Singh, M.* (2020) Structure specific recognition of telomere repeats containing RNA by the RGG-box of hnRNPA1. Nucleic Acids Research. May 7;48(8):4492-4506. doi: 10.1093/nar/gkaa134.
- Ghosh, M. and Singh, M.* (2018) RGG-box in hnRNPA1 specifically recognizes the telomere G-quadruplex DNA and enhances the G-quadruplex unfolding ability of UP1 domain. Nucleic Acids Research. Nov 2. 46(19):10246-10261. doi: 10.1093/nar/gky854.
- Singh, M., Wang, Z., Koo, B.K., Patel, A., Cascio, D., Collins, K. and Feigon, J.* (2012) Structural basis for telomerase RNA recognition and RNP assembly by the holoenzyme La family protein p65. Molecular cell. 47, 16-26.
Please see ‘Publications’ page for the complete list of publications.
Please see ‘Research’ page for more information on current projects in the lab.
